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Publications

38. Late-stage Minimal Labeling of Peptides and Proteins for Real-time Imaging of Cellular Trafficking

Ferran Nadal-Bufi,+ Raj V. Nithun,+ Fabio de Moliner, Xiaxi Lin, Shaimaa Habiballah, Muhammad Jbara,* Marc Vendrell*

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36. A Versatile Method for Site-Specific Chemical Installation of Aromatic Posttranslational Modification Analogs into Proteins

Xiaoxi Lin, Shaswati Mandal, Raj V. Nithun, Rajasekhar Kolla, Bouchra Bouri, Hilal A. Lashuel,* and Muhammad Jbara*

Journal of the American Chemical Society, 2024, 146, 37, 25788–25798

https://pubs.acs.org/doi/10.1021/jacs.4c08416​

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37. Unprecedented Photoinduced-Electron-Transfer Probe with a Turn-ON Chemiluminescence Mode-of-Action

Maya David+, Sara Gutkin+, Raj V. Nithun, Muhammad Jbara,* and Doron Shabat*

Angewandte Chemie International Edition, 2024, 63, e202417924

(Selected as a Very Important Paper)

https://onlinelibrary.wiley.com/doi/10.1002/anie.202417924

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35. Deciphering the Dynamic Code: DNA Recognition by Transcription Factors in the Ever-Changing Genome

Yumi Minyi Yao, Irina Miodownik, Michael O'Hagan, Muhammad Jbara*, Ariel Afek*

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34. Site-specific Acetylation of the Transcription Factor Protein Max Modulates its DNA Binding Activity

Raj V. Nithun,+ Yumi Minyi Yao,+ Omer Harel, Shaimaa Habiballah, Ariel Afek,* and Muhammad Jbara*

ACS Central Science, 2024, 10, 6, 1295–1303

https://pubs.acs.org/doi/epdf/10.1021/acscentsci.4c00686

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33. Chemical Engineering of Artificial Transcription Factors by Orthogonal Palladium(II)-Mediated S-Arylation Reactions

Xiaoxi Lin, Omer Harel and Muhammad Jbara*

Angewandte Chemie International Edition, 2024, 63, e202317511

(Selected as a Hot Paper)

onlinelibrary.wiley.com/doi/10.1002/anie.202317511

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32. Deciphering the Role of the Ser-Phosphorylation Pattern on the DNA-Binding Activity of Max Transcription Factor Using Chemical Protein Synthesis

Raj V. Nithun, Yumi Minyi Yao, Xiaoxi Lin, Shaimaa Habiballah, Ariel Afek*, and Muhammad Jbara*

Angewandte Chemie International Edition, 2023, 62, e202310913

(Selected as a Very Important Paper)

(Highlighted as a Front Cover)

(Highlighted in Doctors & TAU-School of Chemistry websites)

onlinelibrary.wiley.com/doi/epdf/10.1002/anie.202310913

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31. Enabling Peptide Ligation at Aromatic Junction Mimics via Native Chemical Ligation and Palladium-Mediated S-Arylation

Xiaoxi Lin, Raj V Nithun, Raju Samanta, Omer Harel, and Muhammad Jbara*

Organic Letters, 2023, 25, 4715–4719

pubs.acs.org/doi/10.1021/acs.orglett.3c01652

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30. Chemical Synthesis of Bioactive Proteins 

Omer Harel and Muhammad Jbara*

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Angewandte Chemie International Edition, 2023, 62, e202217716

doi.org/10.1002/anie.202217716

29. Histone H3 Serine-57 is a CHK1 Substrate Whose Phosphorylation Affects DNA Repair

Nikolaos Parisis, Pablo Dans Puigrὸs, Muhammad Jbara, Balveer Singh, Diane Schausi-Tiffoche, Diego Molina-Serrano, Isabelle Brun Heath, Denisa Hendrychova, Suman Kumar Maity, Diana Buitrago, Rafael Lema, Thiziri Nait Achour, Simona Giunta, Michael Girardot, Nicolas Talarek, Valérie Rofidal, Katerina Danezi, Damien Coudreuse, Marie-Noëlle Prioleau, Robert Feil, Modesto Orozco, Ashraf Brik, Pei-Yun Jenny Wu, Liliana Krasinska, Daniel Fisher*

Nature Communications, 2023, 14, 5104

https://www.nature.com/articles/s41467-023-40843-4

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28. Peptide and Protein Chemistry: Design, Synthesis, and Applications

Muhammad Jbara

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27. Posttranslational Chemical Mutagenesis Methods to Insert Posttranslational Modifications into Recombinant Proteins

Omer Harel and Muhammad Jbara*

Molecules, 2022, 27(14), 4389

(Invited Paper)

https://www.mdpi.com/1420-3049/27/14/4389

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26. Transition Metal Catalyzed Site-Selective Cysteine Diversification of Proteins

Muhammad Jbara* 

Pure and Applied Chemistry, 2021, 93, 169–186

(Invited Paper)

doi.org/10.1515/pac-2020-0504

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25. Engineering Bioactive Dimeric Transcription Factor Analogs via Palladium Rebound Reagents

Muhammad Jbara,† Sebastian Pomplun,† Carly K Schissel, Susana W Hawken, Ann Boija, Isaac Klein, Jacob Rodriguez, Stephen L Buchwald, and Bradley L Pentelute*

Journal of the American Chemical Society, 2021, 143, 11788–11798

doi.org/10.1021/jacs.1c05666

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24. Oligonucleotide Bioconjugation with Bifunctional Palladium Reagents

Muhammad Jbara, Jacob Rodriguez, Heemal H Dhanjee, Andrei Loas, Stephen L Buchwald,* and Bradley L Pentelute*

Angewandte Chemie International Edition, 2021, 60, 12109-12115

(Selected by the Editor as a Hot Paper)

doi.org/10.1002/anie.202103180

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23. Parallel automated flow synthesis of covalent protein complexes that can inhibit MYC-driven transcription

Sebastian Pomplun,† Muhammad Jbara,† Carly K Schissel, Susana W Hawken, Ann Boija, Charles Li, Isaac Klein, and Bradley L Pentelute*

ACS Central Science, 2021, 7, 8, 1408-1418

(Highlighted as Supplementary Cover)

doi.org/10.1021/acscentsci.1c00663

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22. De Novo Discovery of High-Affinity Peptide Binders for the SARS-CoV-2 Spike Protein

Sebastian Pomplun, Muhammad Jbara, Anthony J Quartararo, Genwei Zhang, Joseph S Brown, Yen-Chun Lee, Xiyun Ye, Stephanie Hanna, and Bradley L Pentelute*

ACS Central Science, 2021, 7, 156–163

doi.org/10.1021/acscentsci.0c01309

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21. Gold(I)-Mediated Decaging or Cleavage of Propargylated Peptide Bond in Aqueous Conditions for Protein Synthesis and Manipulation

Muhammad Jbara,† Emad Eid,† and Ashraf Brik*

Journal of the American Chemical Society, 2020, 142, 8203–8210

Journal of the American Chemical Society, 2020, 142, 8203–8210

doi.org/10.1021/jacs.9b13216

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20. Examining Several Strategies for the Synthesis of Phosphorylated Histone H3 Reveals the Effectiveness of the Convergent Approach

Muhammad Jbara, Suman K Maity, and Ashraf Brik*

European Journal of Organic Chemistry, 2020, 21, 3128-3132

(Selected by the Editor as a Very Important Paper)

doi.org/10.1002/ejoc.201900257

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19. Semi-synthesis of Ubiquitinated Histone H2B with a Native or Non-hydrolyzable Linkage

Michael Morgan, Muhammad Jbara, Ashraf Brik, and Cynthia Wolberger* 

Methods in Enzymology, 2019, 618, 1-27

doi.org/10.1016/bs.mie.2019.01.003

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18. FACT and Ubp10 Collaborate to Modulate H2B Deubiquitination and Nucleosome Dynamics

Melesse Nune, Michael T Morgan, Zaily Connell, Laura McCullough, Muhammad Jbara, Hao Sun, Ashraf Brik, Tim Formosa*, and Cynthia Wolberger*

eLife, 2019, 8, e40988

10.7554/eLife.40988

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17. Chemical Chromatin Ubiquitylation

Muhammad Jbara, Hao Sun, Guy Kamnesky, and Ashraf Brik* 

Current Opinion in Chemical Biology, 2018, 45, 18–26

doi.org/10.1016/j.cbpa.2018.02.001

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16. Palladium Mediated Deallylation in Fully Aqueous Conditions for Native Chemical Ligation at Aspartic and Glutamic Acid Sites

Muhammad Jbara, Emad Eid, and Ashraf Brik* 

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Organic & Biomolecular Chemistry, 2018, 16, 4061-4064

doi.org/10.1039/C8OB00890F

15. Palladium Prompted On-Demand Orthogonal Cysteine Chemistry for the Synthesis of Challenging and Uniquely Modified Proteins

Muhammad Jbara,† Shay Laps,† Michael Morgan, Guy Kamnesky, Guy Mann, Cynthia Wolberger, and Ashraf Brik*

Nature Communications, 2018, 9, 3154

(Featured in Nature Communications Editors' Highlights)

10.1038/s41467-018-05628-0

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14. Total Chemical Synthesis of Methylated Analogues of Histone 3 Revealed KDM4D as a Potential Regulator of H3K79me3

Muhammad Jbara, Noga Guttmann-Raviv, Suman K Maity, Nabieh Ayoub*, and Ashraf Brik*

Bioorganic & Medicinal Chemistry, 2017, 18, 4966-4970

doi.org/10.1016/j.bmc.2017.04.015

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13. Palladium in the Chemical Synthesis and Modification of Proteins

Muhammad Jbara, Suman K Maity, and Ashraf Brik*

Angewandte Chemie International Edition, 2017, 56, 10644–10655

doi.org/10.1002/anie.201702370

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12. Total Chemical Synthesis of Histones and their Analogues Assisted by Native Chemical Ligation and Palladium Complexes

Suman K Maity, Muhammad Jbara, Guy Mann, Guy Kamnesky, and Ashraf Brik*

Nature Protocols, 2017, 12, 2293–2322

10.1038/nprot.2017.049

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11. Palladium Assisted Cleavage of Peptides and Proteins Containing a Backbone Thiazolidine Linkage

Muhammad Jbara, Shay Laps, Suman K Maity, and Ashraf Brik*

Chemistry–A European Journal, 2016, 22, 14851–14855

(Selected by the Editor as a Hot Paper)

doi.org/10.1002/chem.201603676

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10. Palladium Assisted Rapid Removal of a Solubilizing Tag from a Cys Side Chain to Facilitate Peptide and Protein Synthesis. 

Suman K Maity, Guy Mann, Muhammad Jbara, Shay Laps, Guy Kamnesky, and Ashraf Brik*

Organic Letters, 2016, 18, 3026–3029

(ACS Editors' Choice)

doi.org/10.1021/acs.orglett.6b01442

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9. Efficient Palladium-Assisted One-Pot Deprotection of (Acetamidomethyl)Cysteine Following Native Chemical Ligation and/or Desulfurization to Expedite Chemical Protein Synthesis

Suman K Maity,† Muhammad Jbara,† Shay Laps, and Ashraf Brik*

Angewandte Chemie International Edition, 2016, 55, 8108–8112

doi.org/10.1002/anie.201603169

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8. Palladium Mediated Rapid Deprotection of N-terminal Cysteine Under Native Chemical Ligation Conditions for the Efficient Preparation of Synthetically Challenging Proteins

Muhammad Jbara,† Suman K Maity,† Mallikanti Seenaiah, and Ashraf Brik*

Journal of the American Chemical Society, 2016, 138, 5069-5075

doi.org/10.1021/jacs.5b13580

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7. Expanding the Chemical Toolbox for the Synthesis of Large and Unique Proteins

Somasekhar Bondalapati,† Muhammad Jbara,† and Ashraf Brik*

Nature Chemistry, 2016, 8, 407-418

10.1038/nchem.2476

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6. Chemical and Semisynthesis of Modified Histones

Suman K Maity, Muhammad Jbara, and Ashraf Brik*

Journal of Peptide Science, 2016, 22, 252-259

doi.org/10.1002/psc.2848

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5. Chemical Synthesis of Phosphorylated Histone H2A at Tyr57 Reveals Insight into the Inhibition Mode of the SAGA Deubiquitinating Module

Muhammad Jbara, Suman K Maity, Michael Morgan, Cynthia Wolberger, and Ashraf Brik*

Angewandte Chemie International Edition, 2016, 55, 4972–4976

doi.org/10.1002/anie.201600638

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4. Convergent vs. Linear Protein Synthesis: The Case of Ubiquitinated and Glycosylated H2B 

Mallikanti Seenaiah, Muhammad Jbara, Sachitanand M Mali, and Ashraf Brik*

Angewandte Chemie International Edition, 2015, 54, 12374-12378

doi.org/10.1002/ange.201503309

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3. The Ubiquitin Ligase KPC1 Promotes Processing of p105 NF-KB1 to p50, Resulting in Strong Tumor Suppression

Yelena Kravtsova-Ivantsiv, Inna Shomer, Victoria Cohen-Kaplan, Berend Snijder, Giulio Superti-Furga, Hedva Gonen, Thomas Sommer, Tamar Ziv, Arie Admon, Inna Naroditsky, Muhammad Jbara, Ashraf Brik, Eli Pikarsky, Yong Tae Kwon, Ilana Doweck, and Aaron Ciechanover*

Cell, 2015, 161, 333–347

doi.org/10.1016/j.cell.2015.03.001

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2. Solid Phase Chemical Ligation Employing a Rink Amide Linker for the Synthesis of Histone H2B Protein

Muhammad Jbara, Mallikanti Seenaiah, and Ashraf Brik* 

Chemical Communications, 2014, 50, 12534-12537

doi.org/10.1039/C4CC06499B

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1. Modifying the Vicinity of the Isopeptide Bond to Reveal Differential Behavior of Ubiquitin Chains with Interacting Proteins: Organic Chemistry Applied to Synthetic Proteins

Najat Haj‐Yahya, Mahmood Haj‐Yahya, Carlos A Castañeda, Liat Spasser, Hosahalli P Hemantha, Muhammad Jbara, Marlin Penner, Aaron Ciechanover, David Fushman, and Ashraf Brik*

Angewandte Chemie International Edition, 2013,  52, 11149-11153

doi.org/10.1002/anie.201306118

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